HGCC
Human Glioma Cell Cultures.

HGCC is a biobanking effort to provide a panel of newly established and characterized GBM cell lines from GBM patient surgical samples, currently holding 48 cell lines. The HGCC cell lines and accompanying data are available as an open resource facilitating accurate modeling of GBM diversity for basic research and drug discovery.

Overview
  • Diagnosis
  • Clinical Data
  • Blood
  •  
  • Markers
  • Gene Expression
  • Genetics
  •  
  • Markers
  • Gene Expression
  • Genetics
  • In vitro properties
  • Tumor initiation
  • Mouse survival
  • Markers
  •  

HGCC Biobank

Generic placeholder image

Glioblastoma and GSCs
From patient to treatment.

The concept of glioma stem cells (GSCs) has attracted a lot of interest, and a glioma-initiating cell bearing stem cell characteristics has been proposed, with the ability to seed new tumors through the capacity to evade chemotherapy and irradiation.

Basic cancer research, including preclinical tumor models and testing of candidate drugs needs optimized in vitro models that better reflect the patient’s disease, including modeling of the cancer stem cell compartment. We have set up a biobanking effort to culture and characterize a new panel of cells, derived from GBM patient biopsies, based on a combination of two validated techniques for culturing GSCs (Xie et al. 2015). Here, we report the establishment, genomic characterization and in vivo validation of 48 patient-derived GBM cell lines cultured under stem cell conditions, and that they represent all four molecular GBM subtypes.

Patient Material

DiagnosisNumber of patientsMean age (span)MaleFemaleNumber of cell linesCell line rate (%)
All glioma10160.7 (21-82)56455447.4
Grade IV83 5355.8
Glioblastoma8262.5 (21-82)47355356.4
Gliosarcoma178100.0
Grade III13 17.7
Anaplastic astrocytoma445.8(29-63)04125
Anaplastic oligodendroglioma952.6(26-73)4500
Grade II500
Oligodendroglioma455.3(42-68)3100
Pleomorphic xanthoastrcytoma1571000

HGCC in Action
Drug Development and Characterization.

A number of studies have been published using HGCC. Here we summarize the cell lines used in each study.

U3002
U3004
U3005
U3008
U3009
U3013
U3016
U3017
U3019
U3020
U3021
U3024
U3027
U3028
U3029
U3031
U3033
U3034
U3035
U3037
U3039
U3042
U3046
U3047
U3048
U3051
U3053
U3054
U3056
U3060
U3062
U3065
U3067
U3068
U3071
U3073
U3078
U3082
U3084
U3085
U3086
U3088
U3101
U3110
U3117
U3118
U3121
U3123
Xie et al.
Savary et al.
Yu et al.
Wee et al.
Babateen et al.
Roy et al.
Segerman et al.
Kundu et al.
Wee et al.
Mayrhofer et al.
Kling et al.
Schmidt et al.
Truvé et al.
Jiang et al.
Ramachandran et al.
Johansson et al.
Pencheva et al.
Fedele et al.
Roy et al.
Niklasson et al.
Pinheiro et al.
Baskaran et al.
Pudelko et al.
Tong et al.
Jones et al.
Bogeas et al.
Goudarzi et al.
Matuszewski et al.
Niklasson et al.
Čančer et al.
Kaffes et al.
Pölönen et al.
Jimenez-Pascual et al.
Munksgaard et al.
Indira Chandran et al.
Xie et al.
Indira Chandran et al.
Maturi et al.
Johansson et al.
Mega et al.
Goranci-Buzhala et al.
Attwood et al.
Grassi et al.
Merisaari et al.
Tu et al.
Sakthikumar et al.
Verheyen et al.
Grassi et al.
Ricci et al.
Dalmo et al.
Xiong et al.
Almstedt et al.
Johard et al.
Cifarelli et al.
Guelfi et al.
Kwak et al.
Dadras et al.
Larsson et al.
Ensenyat-Mendez et al.
Freire-Benéitez et al.
Guo et al.
Sharma et al.
McKelvey et al.
Masoumi et al.
Almstedt et al.
Filppu et al.
Vinel et al.
Caja et al.
Cerezo-Magaña et al.
Goranci et al.
Berg et al.
Tanabe et al.

Supported By

References

The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes.Xie, Yuan; Bergström, Tobias; Jiang, Yiwen; Johansson, Patrik; Marinescu, Voichita Dana; Lindberg, Nanna; Segerman, Anna; Wicher, Grzegorz; Niklasson, Mia; Baskaran, Sathishkumar; Sreedharan, Smitha; Everlien, Isabelle; Kastemar, Marianne; Hermansson, Annika; Elfineh, Lioudmila; Libard, Sylwia; Holland, Eric Charles; Hesselager, Göran; Alafuzoff, Irina; Westermark, Bengt; Nelander, Sven; Forsberg-Nilsson, Karin; Uhrbom, Lene
EBioMedicine 2, 1351–63 (2015).
U3002, U3004, U3005, U3008, U3009, U3013, U3016, U3017, U3019, U3020, U3021, U3024, U3027, U3028, U3029, U3031, U3033, U3034, U3035, U3037, U3039, U3042, U3046, U3047, U3048, U3051, U3053, U3054, U3056, U3060, U3062, U3065, U3067, U3068, U3071, U3073, U3078, U3082, U3084, U3085, U3086, U3088, U3101, U3110, U3117, U3118, U3121, U3123
Snail depletes the tumorigenic potential of glioblastoma.Savary, K.; Caglayan, D.; Caja, L.; Tzavlaki, K.; Bin Nayeem, S.; Bergström, T.; Jiang, Y.; Uhrbom, L.; Forsberg-Nilsson, K.; Westermark, B.; Heldin, C. H.; Ferletta, M.; Moustakas, A.
Oncogene 32(47), 5409-20 (2013).
U3028, U3034
Adenovirus serotype 5 vectors with Tat-PTD modified hexon and serotype 35 fiber show greatly enhanced transduction capacity of primary cell cultures.Yu, Di; Jin, Chuan; Ramachandran, Mohanraj; Xu, Jing; Nilsson, Berith; Korsgren, Olle; Le Blanc, Katarina; Uhrbom, Lene; Forsberg-Nilsson, Karin; Westermark, Bengt; Adamson, Rachel; Maitland, Norman; Fan, Xiaolong; Essand, Magnus
PLoS ONE 8, e54952 (2013).
U3013, U3054
Selective calcium sensitivity in immature glioma cancer stem cells.Wee, Shimei; Niklasson, Maria; Marinescu, Voichita Dana; Segerman, Anna; Schmidt, Linnéa; Hermansson, Annika; Dirks, Peter; Forsberg-Nilsson, Karin; Westermark, Bengt; Uhrbom, Lene; Linnarsson, Sten; Nelander, Sven; Andäng, Michael
PLoS One 9, e115698 (2014).
U3047, U3013, U3065, U3037, U3035, U3024, U3084, U3046, U3017
Etomidate, propofol and diazepam potentiate GABA-evoked GABAA currents in a cell line derived from human glioblastoma.Babateen, Omar; Jin, Zhe; Bhandage, AmolK; Korol, Sergiy V.; Westermark, Bengt; Forsberg Nilsson, Karin; Uhrbom, Lene; Smits, Anja; Birnir, Bryndis
Eur J Pharmacol 748, 101–7 (2015).
U3047
Glioma-derived plasminogen activator inhibitor-1 (PAI-1) regulates the recruitment of LRP1 positive mast cells.Roy, Ananya; Coum, Antoine; Marinescu, Voichita D.; Põlajeva, Jelena; Smits, Anja; Nelander, Sven; Uhrbom, Lene; Westermark, Bengt; Forsberg-Nilsson, Karin; Pontén, Fredrik; Tchougounova, Elena
Oncotarget 6, 23647–61 (2015).
U3016, U3047, U3117, U3065, U3060, U3062, U3101
Clonal Variation in Drug and Radiation Response among Glioma-Initiating Cells Is Linked to Proneural-Mesenchymal Transition.Segerman, Anna; Niklasson, Mia; Haglund, Caroline; Bergström, Tobias; Jarvius, Malin; Xie, Yuan; Westermark, Ann; Sönmez, Demet; Hermansson, Annika; Kastemar, Marianne; Naimaie-Ali, Zeinab; Nyberg, Frida; Berglund, Malin; Sundström, Magnus; Hesselager, Göran; Uhrbom, Lene; Gustafsson, Mats; Larsson, Rolf; Fryknäs, Mårten; Segerman, Bo; Westermark, Bengt
Cell Rep 17, 2994–3009 (2016).
U3017, U3065, U3117, U3118, U3121, U3167
Heparanase Promotes Glioma Progression and Is Inversely Correlated with Patient Survival.Kundu, Soumi; Xiong, Anqi; Spyrou, Argyris; Wicher, Grzegorz; Marinescu, Voichita D.; Edqvist, Per-Henrik D.; Zhang, Lei; Essand, Magnus; Dimberg, Anna; Smits, Anja; Ilan, Neta; Vlodavsky, Israel; Li, Jin-Ping; Forsberg-Nilsson, Karin
Mol Cancer Res 14, 1243–1253 (2016).
U3013, U3024
ABCG2 regulates self-renewal and stem cell marker expression but not tumorigenicity or radiation resistance of glioma cells.Wee, Boyoung; Pietras, Alexander; Ozawa, Tatsuya; Bazzoli, Elena; Podlaha, Ondrej; Antczak, Christophe; Westermark, Bengt; Nelander, Sven; Uhrbom, Lene; Forsberg-Nilsson, Karin; Djaballah, Hakim; Michor, Franziska; Holland, Eric C.
Sci Rep 6, 25956 (2016).
U3020
Rawcopy: Improved copy number analysis with Affymetrix arrays.Mayrhofer, Markus; Viklund, Björn; Isaksson, Anders
Sci Rep 6, 36158 (2016).
HGCC data used
Integrative Modeling Reveals Annexin A2-mediated Epigenetic Control of Mesenchymal Glioblastoma.Kling, Teresia; Ferrarese, Roberto; Ó hAilín, Darren; Johansson, Patrik; Heiland, Dieter Henrik; Dai, Fangping; Vasilikos, Ioannis; Weyerbrock, Astrid; Jörnsten, Rebecka; Carro, Maria Stella; Nelander, Sven
EBioMedicine 12, 72–85 (2016).
U3047
Case-specific potentiation of glioblastoma drugs by pterostilbene.Schmidt, Linnéa; Baskaran, Sathishkumar; Johansson, Patrik; Padhan, Narendra; Matuszewski, Damian; Green, Lydia C.; Elfineh, Ludmila; Wee, Shimei; Häggblad, Maria; Martens, Ulf; Westermark, Bengt; Forsberg-Nilsson, Karin; Uhrbom, Lene; Claesson-Welsh, Lena; Andäng, Michael; Sintorn, Ida-Maria; Lundgren, Bo; Lönnstedt, Ingrid; Krona, Cecilia; Nelander, Sven
Oncotarget 7, 73200–73215 (2016).
U3013, U3017, U3024, U3037, U3065, U3084, U3002, U3004, U3005, U3009, U3020, U3035, U3008, U3016, U3021, U3027, U3031, U3046, U3029, U3028, U3033, U3039, U3047, U3085, U3019, U3034, U3056, U3082, U3086, U3117, U3053, U3054, U3060, U3073, U3078, U3088, U3051, U3062, U3068, U3110, U3118, U3129
Utilizing the Dog Genome in the Search for Novel Candidate Genes Involved in Glioma Development-Genome Wide Association Mapping followed by Targeted Massive Parallel Sequencing Identifies a Strongly Associated Locus.Truvé, Katarina; Dickinson, Peter; Xiong, Anqi; York, Daniel; Jayashankar, Kartika; Pielberg, Gerli; Koltookian, Michele; Murén, Eva; Fuxelius, Hans-Henrik; Weishaupt, Holger; Swartling, Fredrik J.; Andersson, Göran; Hedhammar, Åke; Bongcam-Rudloff, Erik; Forsberg-Nilsson, Karin; Bannasch, Danika; Lindblad-Toh, Kerstin
PLoS Genet 12, e1006000 (2016).
U3004, U3009, U3013, U3021, U3029, U3031, U3054
Glioblastoma Cell Malignancy and Drug Sensitivity Are Affected by the Cell of Origin.Jiang, Yiwen; Marinescu, Voichita Dana; Xie, Yuan; Jarvius, Malin; Maturi, Naga Prathyusha; Haglund, Caroline; Olofsson, Sara; Lindberg, Nanna; Olofsson, Tommie; Leijonmarck, Caroline; Hesselager, Göran; Alafuzoff, Irina; Fryknäs, Mårten; Larsson, Rolf; Nelander, Sven; Uhrbom, Lene
Cell Rep 18, 977–990 (2017).
U3002, U3005, U3008, U3009, U3013, U3017, U3020, U3021, U3024, U3027, U3028, U3029, U3031, U3033, U3034, U3035, U3037, U3039, U3042, U3046, U3047, U3048, U3051, U3053, U3056, U3060, U3062, U3065, U3068, U3071, U3073, U3078, U3082, U3084, U3085, U3086, U3088, U3093, U3101, U3102, U3104, U3110, U3117, U3118, U3121, U3123, U3129, U3137, U3140, U3146, U3155, U3164, U3167, U3169, U3172, U3173, U3175, U3180, U3189, U3198, U3202
Safe and Effective Treatment of Experimental Neuroblastoma and Glioblastoma Using Systemically Delivered Triple MicroRNA-Detargeted Oncolytic Semliki Forest Virus.Ramachandran, M.; Yu, D.; Dyczynski, M.; Baskaran, S.; Zhang, L.; Lulla, A.; Lulla, V.; Saul, S.; Nelander, S.; Dimberg, A.; Merits, A.; Leja-Jarblad, J.; Essand, M.
Clin Cancer Res 23, 1519–1530 (2017).
U3013, U3016, U3021, U3024, U3028, U3034, U3053, U3054, U3065, U3101, U3213
CD44 Interacts with HIF-2_ to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells.Johansson, E.; Grassi, E. S.; Pantazopoulou, V.; Tong, B.; Lindgren, D.; Berg, T. J.; Pietras, E. J.; Axelson, H.; Pietras, A.
Cell Rep 20, 1641–1653 (2017).
U3035, U3065, U3082, U3084
Identification of a Druggable Pathway Controlling Glioblastoma InvasivenessPencheva, Nora; de Gooijer, Mark C.; Vis, Daniel J.; Wessels, Lodewyk F. A.; Würdinger, Tom; van Tellingen, Olaf; Bernards, René
Cell Reports 20, 48–60 (2017).
U3013, U3017, U3024, U3031, U3054, U3084, U3086, U3117
Epigenetic Regulation of ZBTB18 Promotes Glioblastoma Progression.Fedele, Vita; Dai, Fangping; Masilamani, Anie P.; Heiland, Dieter H.; Kling, Eva; Gätjens-Sanchez, Ana M.; Ferrarese, Roberto; Platania, Leonardo; Soroush, Doostkam; Kim, Hyunsoo; Nelander, Sven; Weyerbrock, Astrid; Prinz, Marco; Califano, Andrea; Iavarone, Antonio; Bredel, Markus; Carro, Maria S.
Mol Cancer Res 15, 998–1011 (2017).
U3047, U3082
Serglycin as a potential biomarker for glioma: association of serglycin expression, extent of mast cell recruitment and glioblastoma progression.Roy, Ananya; Attarha, Sanaz; Weishaupt, Holger; Edqvist, Per-Henrik; Swartling, Fredrik J.; Bergqvist, Michael; Siebzehnrubl, Florian A.; Smits, Anja; Pontén, Fredrik; Tchougounova, Elena
Oncotarget 8, 24815–24827 (2017).
U3017, U3019, U3035, U3039, U3042, U3047, U3054, U3071, U3078, U3084, U3086, U3117
Membrane-Depolarizing Channel Blockers Induce Selective Glioma Cell Death by Impairing Nutrient Transport and Unfolded Protein/Amino Acid ResponsesNiklasson, Mia; Maddalo, Gianluca; Sramkova, Zuzana; Mutlu, Ercan; Wee, Shimei; Sekyrova, Petra; Schmidt, Linnéa; Fritz, Nicolas; Dehnisch, Ivar; Kyriatzis, Gregorios; Krafcikova, Michaela; Carson, Brittany B.; Feenstra, Jennifer M.; Marinescu, Voichita D.; Segerman, Anna; Haraldsson, Martin; Gustavsson, Anna-Lena; Hammarström, Lars G.J.; Jenmalm Jensen, Annika; Uhrbom, Lene; Altelaar, A.F. Maarten; Linnarsson, Sten; Uhlén, Per; Trantirek, Lukas; Vincent, C. Theresa; Nelander, Sven; Enger, Per Øyvind; Andäng, Michael
Cancer Res 77, 1741–1752 (2017).
U3002, U3004, U3005, U3008, U3009, U3013, U3016, U3017, U3019, U3020, U3021, U3024, U3027, U3028, U3029, U3031, U3033, U3034, U3035, U3037, U3039, U3046, U3047, U3051, U3053, U3054, U3056, U3060, U3062, U3065, U3068, U3073, U3078, U3082, U3084, U3085, U3086, U3088, U3101, U3110, U3117, U3118, U3121, U3123, U3129
Reprint of: A chemical screen identifies trifluoperazine as an inhibitor of glioblastoma growth.Pinheiro, T.; Otrocka, M.; Seashore-Ludlow, B.; Rraklli, V.; Holmberg, J.; Forsberg-Nilsson, K.; Simon, A.; Kirkham, M.
Biochem Biophys Res Commun 499, 136–142 (2018).
U3005, U3028, U3046
Primary glioblastoma cells for precision medicine: a quantitative portrait of genomic (in) stability during the first 30 passagesBaskaran, Sathishkumar; Mayrhofer, Markus; Kultima, Hanna Göransson; Bergström, Tobias; Elfineh, Lioudmila; Cavelier, Lucia; Isaksson, Anders; Nelander, Sven
Neuro-oncology (2018) doi:10.1093/neuonc/noy024.
U3021, U3028, U3088
An orthotopic glioblastoma animal model suitable for high-throughput screenings.Pudelko, L.; Edwards, S.; Balan, M.; Nyqvist, D.; Al-Saadi, J.; Dittmer, J.; Almlöf, I.; Helleday, T.; Bräutigam, L.
Neuro Oncol (2018) doi:10.1093/neuonc/noy071.
U3024, U3101
The p75 neurotrophin receptor enhances HIF-dependent signaling in glioma.Tong, B.; Pantazopoulou, V.; Johansson, E.; Pietras, A.
Exp Cell Res 371, 122–129 (2018).
U3020, U3065, U3082, U3084
Analysis of copy number loss of the ErbB4 receptor tyrosine kinase in glioblastoma.Jones, DeAnalisa C.; Scanteianu, Adriana; DiStefano, Matthew; Bouhaddou, Mehdi; Birtwistle, Marc R.
PLoS One 13, e0190664 (2018).
HGCC data used
Changes in chromatin state reveal ARNT2 at a node of a tumorigenic transcription factor signature driving glioblastoma cell aggressiveness.Bogeas, Alexandra; Morvan-Dubois, Ghislaine; El-Habr, Elias A.; Lejeune, François-Xavier; Defrance, Matthieu; Narayanan, Ashwin; Kuranda, Klaudia; Burel-Vandenbos, Fanny; Sayd, Salwa; Delaunay, Virgile; Dubois, Luiz G.; Parrinello, Hugues; Rialle, Stéphanie; Fabrega, Sylvie; Idbaih, Ahmed; Haiech, Jacques; Bièche, Ivan; Virolle, Thierry; Goodhardt, Michele; Chneiweiss, Hervé; Junier, Marie-Pierre
Acta Neuropathol 135, 267–283 (2018).
HGCC data used
Reduced expression of PROX1 transitions glioblastoma cells into a mesenchymal gene expression subtypeGoudarzi, Kaveh M; Espinoza, Jaime A; Guo, Min; Bartek, Jiri; Nistér, Monica; Lindström, Mikael S; Hägerstrand, Daniel
Cancer Res (2018) doi:10.1158/0008-5472.CAN-18-0320.
HGCC data used
Image-Based Detection of Patient-Specific Drug-Induced Cell-Cycle Effects in GlioblastomaMatuszewski, Damian J.; Wählby, Carolina; Krona, Cecilia; Nelander, Sven; Sintorn, Ida-Maria
SLAS DISCOVERY: Advancing the Science of Drug Discovery 23, 1030–1039 (2018).
U3004, U3020, U3029, U3033, U3035, U3047, U3051, U3054, U3073, U3078, U3084, U3086, U3117, U3118, U3129
Mesenchymal transition and increased therapy resistance of glioblastoma cells is related to astrocyte reactivityNiklasson, Mia; Bergström, Tobias; Jarvius, Malin; Sundström, Anders; Nyberg, Frida; Haglund, Caroline; Larsson, Rolf; Westermark, Bengt; Segerman, Bo; Segerman, Anna
The Journal of Pathology 249, 295–307 (2019).
U3017, U3065, U3117, U3118, U3121, U3167
BET and Aurora Kinase A inhibitors synergize against MYCN-positive human glioblastoma cells.Čančer, Matko; Drews, Lisa F.; Bengtsson, Johan; Bolin, Sara; Rosén, Gabriela; Westermark, Bengt; Nelander, Sven; Forsberg-Nilsson, Karin; Uhrbom, Lene; Weishaupt, Holger; Swartling, Fredrik J.
Cell Death Dis 10, 881 (2019).
U3009, U3024, U3054, U3056
Human Mesenchymal glioblastomas are characterized by an increased immune cell presence compared to Proneural and Classical tumors.Kaffes, Ioannis; Szulzewsky, Frank; Chen, Zhihong; Herting, Cameron J.; Gabanic, Ben; Velázquez Vega, José E.; Shelton, Jennifer; Switchenko, Jeffrey M.; Ross, James L.; McSwain, Leon F.; Huse, Jason T.; Westermark, Bengt; Nelander, Sven; Forsberg-Nilsson, Karin; Uhrbom, Lene; Maturi, Naga Prathyusha; Cimino, Patrick J.; Holland, Eric C.; Kettenmann, Helmut; Brennan, Cameron W.; Brat, Daniel J.; Hambardzumyan, Dolores
Oncoimmunology 8, e1655360 (2019).
U3004, U3039, U3042, U3065, U3086, U3088
Nrf2 and SQSTM1/p62 jointly contribute to mesenchymal transition and invasion in glioblastoma.Pölönen, Petri; Jawahar Deen, Ashik; Leinonen, Hanna M.; Jyrkkänen, Henna-Kaisa; Kuosmanen, Suvi; Mononen, Mimmi; Jain, Ashish; Tuomainen, Tomi; Pasonen-Seppänen, Sanna; Hartikainen, Jaana M.; Mannermaa, Arto; Nykter, Matti; Tavi, Pasi; Johansen, Terje; Heinäniemi, Merja; Levonen, Anna-Liisa
Oncogene 38, 7473–7490 (2019).
HGCC data used
ADAMDEC1 Maintains a Growth Factor Signaling Loop in Cancer Stem Cells.Jimenez-Pascual, Ana; Hale, James S.; Kordowski, Anja; Pugh, Jamie; Silver, Daniel J.; Bayik, Defne; Roversi, Gustavo; Alban, Tyler J.; Rao, Shilpa; Chen, Rui; McIntyre, Thomas M.; Colombo, Giorgio; Taraboletti, Giulia; Holmberg, Karl O.; Forsberg-Nilsson, Karin; Lathia, Justin D.; Siebzehnrubl, Florian A.
Cancer Discov 9, 1574–1589 (2019).
U3019
Integrin _10, a Novel Therapeutic Target in Glioblastoma, Regulates Cell Migration, Proliferation, and Survival.Munksgaard Thorén, Matilda; Chmielarska Masoumi, Katarzyna; Krona, Cecilia; Huang, Xiaoli; Kundu, Soumi; Schmidt, Linnéa; Forsberg-Nilsson, Karin; Floyd Keep, Marcus; Englund, Elisabet; Nelander, Sven; Holmqvist, Bo; Lundgren-Åkerlund, Evy
Cancers (Basel) 11, (2019).
U3046, U3054, U3071, U3073, U3071
Global extracellular vesicle proteomic signature defines U87-MG glioma cell hypoxic status with potential implications for non-invasive diagnosticsIndira Chandran, Vineesh; Welinder, Charlotte; Gonçalves de Oliveira, Kelin; Cerezo-Magaña, Myriam; Månsson, Ann-Sofie; Johansson, Maria C.; Marko-Varga, Gyorgy; Belting, Mattias
J Neurooncol 144, 477–488 (2019).
U3046, U3054, U3071, U3073, U3071
LGR5 promotes tumorigenicity and invasion of glioblastoma stem-like cells and is a potential therapeutic target for a subset of glioblastoma patients: LGR5 regulates glioblastoma tumorigenicity and invasionXie, Yuan; Sundström, Anders; Maturi, Naga P; Tan, E-Jean; Marinescu, Voichita D; Jarvius, Malin; Tirfing, Malin; Jin, Chuan; Chen, Lei; Essand, Magnus; Swartling, Fredrik J; Nelander, Sven; Jiang, Yiwen; Uhrbom, Lene
J. Pathol. 247, 228–240 (2019).
U3002, U3005, U3008, U3009, U3013, U3017, U3020, U3021, U3024, U3027, U3028, U3029, U3031, U3033, U3034, U3035, U3037, U3039, U3042, U3046, U3047, U3048, U3051, U3053, U3056, U3060, U3062, U3065, U3068, U3071, U3073, U3078, U3082, U3084, U3085, U3086, U3088, U3093, U3101, U3102, U3104, U3110, U3117, U3118, U3121, U3123, U3129, U3137, U3140, U3146, U3155, U3164, U3167, U3169, U3172, U3173, U3180, U3189, U3198, U3202
Ultrasensitive Immunoprofiling of Plasma Extracellular Vesicles Identifies Syndecan-1 as a Potential Tool for Minimally Invasive Diagnosis of GliomaIndira Chandran, Vineesh; Welinder, Charlotte; Månsson, Ann-Sofie; Offer, Svenja; Freyhult, Eva; Pernemalm, Maria; Lund, Sigrid M.; Pedersen, Shona; Lehtiö, Janne; Marko-Varga, Gyorgy; Johansson, Maria C.; Englund, Elisabet; Sundgren, Pia C.; Belting, Mattias
Clin Cancer Res 25, 3115–3127 (2019).
U3034
A molecularly distinct subset of glioblastoma requires serum-containing media to establish sustainable bona fide glioblastoma stem cell culturesMaturi, Naga Prathyusha; Tan, E.-Jean; Xie, Yuan; Sundström, Anders; Bergström, Tobias; Jiang, Yiwen; Uhrbom, Lene
Glia 68, 1228–1240 (2020).
U3002, U3005, U3008, U3009, U3013, U3017, U3020, U3021, U3024, U3027, U3028, U3029, U3031, U3033, U3034, U3035, U3037, U3039, U3042, U3046, U3047, U3048, U3049, U3051, U3053, U3054, U3056, U3060, U3062, U3065, U3068, U3071, U3073, U3078, U3082, U3084, U3085, U3088, U3093, U3102, U3104, U3110, U3117, U3121, U3123
A Patient-Derived Cell Atlas Informs Precision Targeting of GlioblastomaJohansson, Patrik; Krona, Cecilia; Kundu, Soumi; Doroszko, Milena; Baskaran, Sathishkumar; Schmidt, Linnéa; Vinel, Claire; Almstedt, Elin; Elgendy, Ramy; Elfineh, Ludmila; Gallant, Caroline; Lundsten, Sara; Ferrer Gago, Fernando J.; Hakkarainen, Aleksi; Sipilä, Petra; Häggblad, Maria; Martens, Ulf; Lundgren, Bo; Frigault, Melanie M.; Lane, David P.; Swartling, Fredrik J.; Uhrbom, Lene; Nestor, Marika; Marino, Silvia; Nelander, Sven
Cell Reports 32, 107897 (2020).
See http://portal.hgcc.se
Astrocytes enhance glioblastoma growthMega, Alessandro; Hartmark Nilsen, Mette; Leiss, Lina Wik; Tobin, Nicholas P.; Miletic, Hrvoje; Sleire, Linda; Strell, Carina; Nelander, Sven; Krona, Cecilia; Hägerstrand, Daniel; Enger, Per Ø; Nistér, Monica; Östman, Arne
Glia 68, 316–327 (2020).
U3013
Rapid and Efficient Invasion Assay of Glioblastoma in Human Brain OrganoidsGoranci-Buzhala, Gladiola; Mariappan, Aruljothi; Gabriel, Elke; Ramani, Anand; Ricci-Vitiani, Lucia; Buccarelli, Mariachiara; D'Alessandris, Quintino Giorgio; Pallini, Roberto; Gopalakrishnan, Jay
Cell Rep 31, 107738 (2020).
U3024, 3047
Raloxifene prevents stress granule dissolution, impairs translational control and promotes cell death during hypoxia in glioblastoma cellsAttwood, Kathleen M.; Robichaud, Aaron; Westhaver, Lauren P.; Castle, Elizabeth L.; Brandman, David M.; Balgi, Aruna D.; Roberge, Michel; Colp, Patricia; Croul, Sidney; Kim, Inhwa; McCormick, Craig; Corcoran, Jennifer A.; Weeks, Adrienne
Cell Death Dis 11, 1–18 (2020).
U3024
Niche-derived soluble DLK1 promotes glioma growth.Grassi, Elisa S.; Jeannot, Pauline; Pantazopoulou, Vasiliki; Berg, Tracy J.; Pietras, Alexander
Neoplasia 22, 689–701 (2020).
U3065, U3082, U3084
Monotherapy efficacy of blood-brain barrier permeable small molecule reactivators of protein phosphatase 2A in glioblastoma.Merisaari, Joni; Denisova, Oxana V.; Doroszko, Milena; Le Joncour, Vadim; Johansson, Patrik; Leenders, William P. J.; Kastrinsky, David B.; Zaware, Nilesh; Narla, Goutham; Laakkonen, Pirjo; Nelander, Sven; Ohlmeyer, Michael; Westermarck, Jukka
Brain Commun 2, fcaa002 (2020).
U3008, U3013, U3017, U30564, U3140, U3173, U3179, U3180, U3213
A facile and scalable in production non-viral gene engineered mesenchymal stem cells for effective suppression of temozolomide-resistant (TMZR) glioblastoma growth.Tu, Geraldine Xue En; Ho, Yoon Khei; Ng, Zhi Xu; Teo, Ke Jia; Yeo, Tseng Tsai; Too, Heng-Phon
Stem Cell Res Ther 11, 391 (2020).
U3008, U3020, U3054, U3117
Whole-genome sequencing of glioblastoma reveals enrichment of non-coding constraint mutations in known and novel genes.Sakthikumar, Sharadha; Roy, Ananya; Haseeb, Lulu; Pettersson, Mats E.; Sundström, Elisabeth; Marinescu, Voichita D.; Lindblad-Toh, Kerstin; Forsberg-Nilsson, Karin
Genome Biol 21, 127 (2020).
Spatial organization-dependent EphA2 transcriptional responses revealed by ligand nanocalipers.Verheyen, Toon; Fang, Trixy; Lindenhofer, Dominik; Wang, Yang; Akopyan, Karen; Lindqvist, Arne; Högberg, Björn; Teixeira, Ana I.
Nucleic Acids Res 48, 5777–5787 (2020).
U3013
Hypoxia-induced release, nuclear translocation, and signaling activity of a DLK1 intracellular fragment in glioma.Grassi, Elisa Stellaria; Pantazopoulou, Vasiliki; Pietras, Alexander
Oncogene 39, 4028–4044 (2020).
U3035, U3046, U3065, U3082, U3084
Polycomb-mediated repression of EphrinA5 promotes growth and invasion of glioblastoma.Ricci, Barbara; Millner, Thomas O.; Pomella, Nicola; Zhang, Xinyu; Guglielmi, Loredana; Badodi, Sara; Ceric, Dario; Gemma, Carolina; Cognolato, Erica; Zhang, Ying; Brandner, Sebastian; Barnes, Michael R.; Marino, Silvia
Oncogene 39, 2523–2538 (2020).
U3033, U3118
Growth-Inhibitory Activity of Bone Morphogenetic Protein 4 in Human Glioblastoma Cell Lines Is Heterogeneous and Dependent on Reduced SOX2 ExpressionDalmo, Erika; Johansson, Patrik; Niklasson, Mia; Gustavsson, Ida; Nelander, Sven; Westermark, Bengt
Mol Cancer Res 18, 981–991 (2020).
U3002, U3004, U3005, U3008, U3009, U3013, U3016, U3017, U3019, U3020, U3021, U3024, U3027, U3028, U3029, U3031, U3033, U3034, U3035, U3037, U3039, U3042, U3046, U3047, U3048, U3051, U3053, U3054, U3056, U3060, U3062, U3065, U3068, U3071, U3073, U3078, U3082, U3084, U3085, U3086, U3088, U3093, U3101, U3102, U3104, U3110, U3117, U3118, U3121, U3123
Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon OverexpressionXiong, Anqi; Roy, Ananya; Spyrou, Argyris; Weishaupt, Holger; Marinescu, Voichita D.; Olofsson, Tommie; Hermanson, Ola; Swartling, Fredrik J.; Forsberg-Nilsson, Karin
Cancers 12, 1483 (2020).
U3013
Integrative discovery of treatments for high-risk neuroblastomaAlmstedt, Elin; Elgendy, Ramy; Hekmati, Neda; Rosén, Emil; Wärn, Caroline; Olsen, Thale Kristin; Dyberg, Cecilia; Doroszko, Milena; Larsson, Ida; Sundström, Anders; Arsenian Henriksson, Marie; Påhlman, Sven; Bexell, Daniel; Vanlandewijck, Michael; Kogner, Per; Jörnsten, Rebecka; Krona, Cecilia; Nelander, Sven
Nat Commun 11, 71 (2020).
U3013
HCN Channel Activity Balances Quiescence and Proliferation in Neural Stem Cells and Is a Selective Target for Neuroprotection During Cancer TreatmentJohard, Helena; Omelyanenko, Anna; Fei, Gao; Zilberter, Misha; Dave, Zankruti; Abu-Youssef, Randa; Schmidt, Linnéa; Harisankar, Aditya; Vincent, C. Theresa; Walfridsson, Julian; Nelander, Sven; Harkany, Tibor; Blomgren, Klas; Andäng, Michael
Mol Cancer Res 18, 1522–1533 (2020).
U3002, U3004, U3005, U3008, U3009, U3013, U3016, U3017, U3019, U3020, U3021, U3024, U3027, U3028, U3029, U3031, U3033, U3034, U3035, U3037, U3039, U3042, U3046, U3047, U3048, U3051, U3053, U3054, U3056, U3060, U3062, U3065, U3068, U3071, U3073, U3078, U3082, U3084, U3085, U3086, U3088, U3093, U3101, U3102, U3104, U3110, U3117, U3118, U3121, U3123
Heterogeneity of radiation response in mesenchymal subtype glioblastoma: molecular profiling and reactive oxygen species generationCifarelli, Christopher P.; Jacques, Angelica; Bobko, Andrey
J Neurooncol 152, 245–255 (2021).
U3020, U3035
SLUG and Truncated TAL1 Reduce Glioblastoma Stem Cell Growth Downstream of Notch1 and Define Distinct Vascular Subpopulations in Glioblastoma Multiforme.Guelfi, Sophie; Orsetti, Béatrice; Deleuze, Virginie; Rigau, Valérie; Bauchet, Luc; Duffau, Hugues; Rothhut, Bernard; Hugnot, Jean-Philippe
Cancers (Basel) 13, (2021).
HGCC data used
Glioblastoma cytotoxicity conferred through dual disruption of endolysosomal homeostasis by Vacquinol-1.Kwak, Dongoh; Hammarström, Lars G. J.; Haraldsson, Martin; Ernfors, Patrik
Neurooncol Adv 3, vdab152 (2021).
U3013, U3024
The polarity protein Par3 coordinates positively self-renewal and negatively invasiveness in glioblastoma.Dadras, Mahsa Shahidi; Caja, Laia; Mezheyeuski, Artur; Liu, Sijia; Gélabert, Caroline; Gomez-Puerto, Maria Catalina; Gallini, Radiosa; Rubin, Carl-Johan; Ten Dijke, Peter; Heldin, Carl-Henrik; Moustakas, Aristidis
Cell Death Dis 12, 932 (2021).
U3005, U3028, U3031, U3034
Modeling glioblastoma heterogeneity as a dynamic network of cell states.Larsson, Ida; Dalmo, Erika; Elgendy, Ramy; Niklasson, Mia; Doroszko, Milena; Segerman, Anna; Jörnsten, Rebecka; Westermark, Bengt; Nelander, Sven
Mol Syst Biol 17, e10105 (2021).
U3017, U3065, U3071
iGlioSub: an integrative transcriptomic and epigenomic classifier for glioblastoma molecular subtypes.Ensenyat-Mendez, Miquel; Íñiguez-Muñoz, Sandra; Sesé, Borja; Marzese, Diego M.
BioData Min 14, 42 (2021).
HGCC data used
Elucidation of the BMI1 interactome identifies novel regulatory roles in glioblastoma.Freire-Benéitez, Verónica; Pomella, Nicola; Millner, Thomas O.; Dumas, Anaëlle A.; Niklison-Chirou, Maria Victoria; Maniati, Eleni; Wang, Jun; Rajeeve, Vinothini; Cutillas, Pedro; Marino, Silvia
NAR Cancer 3, zcab009 (2021).
U3082, U3118
SFRP2 induces a mesenchymal subtype transition by suppression of SOX2 in glioblastoma.Guo, Min; Goudarzi, Kaveh M.; Abedi, Shiva; Pieber, Melanie; Sjöberg, Elin; Behnan, Jinan; Zhang, Xing-Mei; Harris, Robert A.; Bartek, Jiri; Lindström, Mikael S.; Nistér, Monica; Hägerstrand, Daniel
Oncogene 40, 5066–5080 (2021).
HGCC data used
Human telomerase is directly regulated by non-telomeric TRF2-G-quadruplex interaction.Sharma, Shalu; Mukherjee, Ananda Kishore; Roy, Shuvra Shekhar; Bagri, Sulochana; Lier, Silje; Verma, Meenakshi; Sengupta, Antara; Kumar, Manish; Nesse, Gaute; Pandey, Deo Prakash; Chowdhury, Shantanu
Cell Rep 35, 109154 (2021).
U3013
Glycolysis and Fatty Acid Oxidation Inhibition Improves Survival in Glioblastoma.McKelvey, Kelly J.; Wilson, Erica B.; Short, Susan; Melcher, Alan A.; Biggs, Michael; Diakos, Connie I.; Howell, Viive M.
Front Oncol 11, 633210 (2021).
HGCC data used
Integrin _10-Antibodies Reduce Glioblastoma Tumor Growth and Cell Migration.Masoumi, Katarzyna Chmielarska; Huang, Xiaoli; Sime, Wondossen; Mirkov, Anna; Munksgaard Thorén, Matilda; Massoumi, Ramin; Lundgren-Åkerlund, Evy
Cancers (Basel) 13, (2021).
U3046, U3054, U3078
Real-time evaluation of glioblastoma growth in patient-specific zebrafish xenograftsAlmstedt, Elin; Rosén, Emil; Gloger, Marleen; Stockgard, Rebecka; Hekmati, Neda; Koltowska, Katarzyna; Krona, Cecilia; Nelander, Sven
Neuro-Oncology noab264 (2021) doi:10.1093/neuonc/noab264.
U3008, U3013, U3017, U3051, U3054, U3137, U3173, U3179, U3180, U3213, U3291
CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activityFilppu, Pauliina; Ramanathan, Jayendrakishore Tanjore; Granberg, Kirsi J.; Gucciardo, Erika; Haapasalo, Hannu; Lehti, Kaisa; Nykter, Matti; Le Joncour, Vadim; Laakkonen, Pirjo
JCI Insight 6, e141486 (2021).
HGCC data used
Comparative epigenetic analysis of tumour initiating cells and syngeneic EPSC-derived neural stem cells in glioblastomaVinel, Claire; Rosser, Gabriel; Guglielmi, Loredana; Constantinou, Myrianni; Pomella, Nicola; Zhang, Xinyu; Boot, James R.; Jones, Tania A.; Millner, Thomas O.; Dumas, Anaelle A.; Rakyan, Vardhman; Rees, Jeremy; Thompson, Jamie L.; Vuononvirta, Juho; Nadkarni, Suchita; El Assan, Tedani; Aley, Natasha; Lin, Yung-Yao; Liu, Pentao; Nelander, Sven; Sheer, Denise; Merry, Catherine L. R.; Marelli-Berg, Federica; Brandner, Sebastian; Marino, Silvia
Nat Commun 12, 6130 (2021).
HGCC data used
The protein kinase LKB1 promotes self_renewal and blocks invasiveness in glioblastomaCaja, Laia; Dadras, Mahsa Shahidi; Mezheyeuski, Artur; Rodrigues_Junior, Dorival Mendes; Liu, Sijia; Webb, Anna Taylor; Gomez_Puerto, Maria Catalina; ten Dijke, Peter; Heldin, Carl_Henrik; Moustakas, Aristidis
J Cell Physiol jcp.30542 (2021) doi:10.1002/jcp.30542.
U3005, U3013, U3017, U3024, U3028, U3031, U3034
Hypoxic Induction of Exosome Uptake through Proteoglycan-Dependent Endocytosis Fuels the Lipid Droplet Phenotype in GliomaCerezo-Magaña, Myriam; Christianson, Helena C.; van Kuppevelt, Toin H.; Forsberg-Nilsson, Karin; Belting, Mattias
Mol Cancer Res 19, 528–540 (2021).
U3034, U3065, U3082
Cilium induction triggers differentiation of glioma stem cellsGoranci-Buzhala, Gladiola; Mariappan, Aruljothi; Ricci-Vitiani, Lucia; Josipovic, Natasa; Pacioni, Simone; Gottardo, Marco; Ptok, Johannes; Schaal, Heiner; Callaini, Giuliano; Rajalingam, Krishnaraj; Dynlacht, Brian; Hadian, Kamyar; Papantonis, Argyris; Pallini, Roberto; Gopalakrishnan, Jay
Cell Reports 36, 109656 (2021).
U3024, U3047, U3056, U3082
The Irradiated Brain Microenvironment Supports Glioma Stemness and Survival via Astrocyte-Derived Transglutaminase 2Berg, Tracy J.; Marques, Carolina; Pantazopoulou, Vasiliki; Johansson, Elinn; von Stedingk, Kristoffer; Lindgren, David; Jeannot, Pauline; Pietras, Elin J.; Bergström, Tobias; Swartling, Fredrik J.; Governa, Valeria; Bengzon, Johan; Belting, Mattias; Axelson, Håkan; Squatrito, Massimo; Pietras, Alexander
Cancer Res 81, 2101–2115 (2021).
U3082
PRRX1 induced by BMP signaling decreases tumorigenesis by epigenetically regulating glioma_initiating cell properties via DNA methyltransferase 3ATanabe, Ryo; Miyazono, Kohei; Todo, Tomoki; Saito, Nobuhito; Iwata, Caname; Komuro, Akiyoshi; Sakai, Satoshi; Raja, Erna; Koinuma, Daizo; Morikawa, Masato; Westermark, Bengt; Heldin, Carl_Henrik
Molecular Oncology 16, 269–288 (2022).
U3005, U3024

Image Attributions

Glioblastoma MRI, Case courtesy of Dr Frank Gaillard, Radiopaedia.org. From the case Glioblastoma multiforme (GBM) with PNET component

Start page background: Intermediate magnification micrograph of a glioblastoma
Copyright © 2011 Nephron
Permission is granted to copy, distribute and/or modify this image under the terms of the GNU Free Documentation License Version 1.2 or any later version published by the Free Software Foundation.